Biodiversity Analysis of Pseudomonas. sp using 16S r-DNA Genomic Technique

Soil microbial diversity is an important index of agricultural productivity by enhancing soil quality. Present study employed the combination of several methodologies to examine genetic relationships among specific groups of bacteria and to characterize strains at the species level using molecular biotyping techniques. The present study is based upon 16SrDNA analysis for characterisation of bacterial species isolated from Raisen, Bhopal, districts of Madhya Pradesh. On the basis of different biochemical test, 75 strains of P. aeroginosa, P.putida, P.flourosence and P.streuzi , strains were confirmed. Genetic diversity analysis of 75 Pseudomonas isolates was done by 16SrDNA-RFLP PCR, and REP PCR Restriction enzyme Alu I, Taq I, Mob I, Hae III, Hind III, Msp I were used for digestion of the amplified 16SrDNA of 75 Pseudomonas isolates.16SrDNA -RFLP PCR, REP PCR of S1 to S50 Pseudomonas isolates was done . Heterogeneity and variation with in species S27 and S38 shows 67% similarity strains S26 shows 77% dissimilarity with rest of the strain. The dendrogram was prepared by using UPGMA method of NTSYS software .The dendrogram shows grouping at 32% similarity and 42% similarity. Strain P3, P7, P9, P12, P15, P20 and P22 showed negligible amount of phosphate solubilization in test media as compared to rest of Pseudomonas sp. All the strains of P.fluorescens and P. aeruginosa showed production of ammonia. Fluorescent Pseudomonad strains reported showed phosphate solubilization potential and combination of Bradyrhizobium species as biofertilizer will serve as potential inoculants in promotion of agronomic applications of biological components specially microbes.